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Journal of Korean Cleft Palate-Craniofacial Association 2002;3(2):113-122.
The Estimation of Osteogenic Potency and Quantitative Analysis of Antibody Titer of Human Bone Xenograft to Rabbit.
Keun Cheol Lee, Ji Hoon Park, Kyoung Oh, Jeong Tae Kim, Seok Kwun Kim
Department of Plastic & Reconstructive Surgery, College of Medicine, Dong-A University, Busan, Korea. sgkim1@daunet.donga.ac.kr
According to the development of craniofacial surgery, various bone grafts were used for the contouring and reconstruction of craniofacial bone defects. Recently, autogenous bone graft has been used widely but there are several drawbacks associated to the autogenous bone grafting. In addition, using allogenic bone has a few problems. Alloplastic materials are expensive and sometimes infected. Therefore, our research is focused on the xenogenic bone graft which has advantage associated with no restriction in harvesting doner bone. The author undertook an experimental investigation as following: Skull and femur defects were made in size of 1x1cm each on 48 New Zealand white rabbits. The defects were grafted with human bone, and experimental group was divided into four groups depending on conditions of doner bone: Group 1 with deep frozen human bone, group 2 with autoclaving human bone, group 3 with deep frozen human bone and immunosuppression with FK-506, group 4 With autoclaving human bone and immunosuppression with FK-506. The specimens were obtained at 1, 2, 4, 8, 16, 24 weeks postoperatively. The author investigated new bone formation by gross appearance, microscopic and radiologic findings in grafted sites and calculated xenogenic antibody titer using ELISA in blood sample. The experimental results are as followings. Neovasculization was shown in connective tissue surrounding the grafted bone and new bone formation was started a week postoperatively and it is significantly increased at 4 weeks postoperatively. At 16 and 24 weeks, new bone formation was more remarkable in group 2 and 4, especially in group 2. Xenogenic antibody titer was higher in group 1 and 3 and immunosuppressed groups with FK-506 showed decreased xenogenic antibody titer. At 24 weeks postoperatively, callus formation and bone union were more remarkable in group 2, 4. According to this experimental studies, the author suggested that new bone formation was enhanced by osteoconductive properties of xenogenic bone and viability of bone was more injured in autoclaved bone than deep frozen bone. And immunologic reaction was suppressed satisfactorily by FK-506.
Keywords: Osteogenic potency; Human bone xenograft
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